Total Protein Indicators

Total protein swabs detect protein residues on equipment surfaces but cannot distinguish between allergenic and non-allergenic proteins. The assumption is that a protein-free surface is likely allergen-free. These swabs are easy to use, sensitive, cost-effective, and practical for controlling multiple allergens.

Kit Contents

Most kits include:

• Swab devices containing a testing liquid
• A waiting buffer (if swabs are not pre-wetted)

Testing Process (3 Steps)

1. Sample Collection
   • Swab the surface with sufficient pressure to maximize sample collection.
   • Avoid touching the swab shaft to prevent contamination.
   • Consistently swab large areas, especially hard-to-clean spots.
2. Test Activation
   • Snap the valve by bending the bulb.
   • Squeeze to release the liquid into the tube and shake for five seconds.
3. Incubation
   • Incubate at 55°C for 15 minutes or 37°C for 30 minutes using a dry incubation block.
   • Compare the liquid color to the provided chart: Green = Clean, Purple = Protein Detected.

Key Considerations

• Total protein swabs do not differentiate allergens—further allergen-specific tests may be needed.
• Alkaline sanitizers may cause false negatives, while peroxide-based disinfectants may cause false positives. Ensure the surface is free of residues before testing.
• Focus on difficult-to-clean areas for accurate assessment of cleaning effectiveness.

Conclusion

Total protein swabs are a reliable, low-cost tool for assessing cleaning efficacy, particularly useful when managing multiple allergens.