microproof® Legionella Quantification LyoKit

The microproof Legionella Quantification LyoKit enables rapid, quantitative detection of Legionella species, L. pneumophila, and L. pneumophila serogroup 1 in water samples using real-time PCR technology. This lyophilized PCR kit offers reliable detection down to 3 GU/reaction and quantification from 10 GU/reaction, meeting ISO/TS 12869:2019 specifications. Perfect for water quality testing in cooling towers, hotels, hospitals, and industrial facilities, the kit includes internal controls and quantification standards for accurate results. Colony confirmation capability makes it a comprehensive solution for food safety and water quality professionals.

Detection:Legionella spp., L. pneumophila, L. pneumophila serogroup 1

Technology: Real-time PCR

Reactions: 96

Product Number:

LP: KIT230119

RP: KIT230120

Key Benefits

Accurate Results

Trust results due to a high sensitivity and specificity

Differentiates closely related species, meaning fewer re-tests and improvement of public health.

Robust Procedure

Streamlined workflows

Minimizes user errors and cross-contamination

Easy-to-Use

Few pipetting steps

Simple sample and reagent handling

Superior Sensitivity

Detects small organism numbers with low DNA levels (or low levels of DNA)

Easily detects low-level contamination.

Broad Compatibility

Suitable for a variety of real-time cyclers

Allows fast integration into existing PCR labs

Multiplex Assays

Detect multiple organisms in one single test

Identify several targets in one assay to save sample volume and time by improving workflows

foodproof® Workflow

The foodproof® workflow delivers highly reliable detection through a systematic three-step approach: enrichment, optimized DNA extraction matched to your sample type and throughput needs, and real-time PCR detection. This standardized process ensures superior accuracy through high-purity DNA extracts, making it ideal for challenging food matrices.

If you are using a Roche LightCycler® 480 with this detection kit use the Color Compensation Set 5.

Sample Enrichment

Specific growth of target organisms via selective media for a reliable detection

Tailored DNA/RNA Extraction

Procedures tailored to your needs to always ensure a high-quality DNA extraction even from complex samples matrices

Extraction

We provide extraction kits tailored to your needs:

Extraction Type:?

foodproof® StarPrep Two Kit

The foodproof StarPrep Two Kit provides rapid DNA extraction from bacteria, yeast, and mold for direct PCR applications. This streamlined kit handles enrichment cultures, direct samples, and filtered water samples, processing up to 1 mL sample volume in a single tube. The lysis buffer eliminates hazardous organic extractions and chaotropic agents while minimizing cross-contamination risks. Designed for 96 reactions, the kit delivers high-quality DNA suitable for PCR detection of Listeria, Clostridium, Legionella, beer-spoiling bacteria, spoilage yeast, Aspergillus and others. Save time with reduced handling steps and get reliable results for food safety testing.

Volume:

42 mL

Product Number: KIT230177

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Additional Reagents

Depending on the extraction protocol, additional reagents and devices are used:

Reagent D

Reagent D eliminates DNA from dead cells to prevent false-positive PCR results in food safety testing. This light-sensitive reagent penetrates dead cell membranes and binds to DNA upon light exposure, blocking PCR amplification. Validated for testing Enterobacteriaceae, Cronobacter, Salmonella, Legionella, and Vibrio in various food matrices including instant milk formula and dairy products. Compatible with Hygiena's DNA isolation kits, Reagent D ensures accurate detection of viable microorganisms in food safety and environmental monitoring applications.

Volume:

Product Number: 15ml: KIT230003

Product Instructions

Reference Guide

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Rinse Buffer

The Rinse Buffer is designed for optimal recovery of microbes from filter membranes.

Volume:

100 mL

Product Number: KIT230012

Reference Guide

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Suspension Buffer

The Suspension Buffer simplifies bacterial colony preparation for PCR testing with a streamlined, single-tube protocol. This specialized buffer enables direct suspension of colonies from agar plates without hazardous organic extractions or chaotropic agents. The extract maintains cell viability for additional analyses like serotyping or DNA sequencing. The efficient process delivers PCR-ready samples while reducing handling steps and exposure to hazardous materials. Perfect for quality control labs needing fast, reliable sample preparation for food safety and microbial identification testing.

Volume:

40 mL

Product Number: KIT230178

Product Instructions

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Real-Time PCR Detection

Assay execution and real-time PCR amplification for clear presence/absence results

Product Library

Filter documents:

CategoryLanguage

Title	Categories	Language
foodproof® and microproof® LyoKits SDS (EN)	Safety Data Sheet	English
microproof Legionella Quantification LyoKit Calculation Worksheet/Template	Worksheet	English
microproof Legionella Quantification LyoKit Instructions (EN)	Instructions	English
microproof Legionella Quantification LyoKit Product Sheet (EN)	Product Sheet	English
microproof Legionella Quantification LyoKit RR Guide	Ready Reference Guides	English

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