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microproof® Legionella Quantification LyoKit

The microproof Legionella Quantification LyoKit enables rapid, quantitative detection of Legionella species, L. pneumophila, and L. pneumophila serogroup 1 in water samples using real-time PCR technology. This lyophilized PCR kit offers reliable detection down to 3 GU/reaction and quantification from 10 GU/reaction, meeting ISO/TS 12869:2019 specifications. Perfect for water quality testing in cooling towers, hotels, hospitals, and industrial facilities, the kit includes internal controls and quantification standards for accurate results. Colony confirmation capability makes it a comprehensive solution for food safety and water quality professionals.

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The microproof Legionella Quantification LyoKit enables rapid, quantitative detection of Legionella species, L. pneumophila, and L. pneumophila serogroup 1 in water samples using real-time PCR technology. This lyophilized PCR kit offers reliable detection down to 3 GU/reaction and quantification from 10 GU/reaction, meeting ISO/TS 12869:2019 specifications. Perfect for water quality testing in cooling towers, hotels, hospitals, and industrial facilities, the kit includes internal controls and quantification standards for accurate results. Colony confirmation capability makes it a comprehensive solution for food safety and water quality professionals.

Legionella spp., L. pneumophila, L. pneumophila serogroup 1
Technology: Real-time PCR
96
KIT230119 (LP), KIT230120 (RP)

Key Benefits

Accurate Results
Trust results due to a high sensitivity and specificity
Differentiates closely related species, meaning fewer re-tests and improvement of public health.
Great Convenience
Few and simple pipetting steps
Easy and less error-prone sample and reagent handling
Easy to Use
Streamlined workflows with simplified steps
Minimizes user errors and cross-contamination.
Superior Sensitivity
Detects small organism numbers with low DNA levels (or low levels of DNA)
Easily detects low-level contamination.
Broad Compatibility
Suitable for a variety of real-time cyclers
Allows fast integration into an already existing PCR lab
Multiplex Assays
Detect multiple organisms in one single test
Identify several targets in one assay to save sample volume and time by improving workflows

foodproof® Workflow

We offer a broad portfolio of products for a robust and reliable real-time PCR detection by providing not only kits tailored to the needs in your lab but also instruments for automation of liquid handling and real-time PCR cyclers.

Sample Enrichment
Workflow next
Tailored DNA/RNA Extraction
Workflow next
Real-Time PCR Detection
If you are using a Roche LightCycler® 480 with this detection kit use the Color Compensation Set 5.

Extraction

We provide extraction kits tailored to your needs:

Extraction Type:?
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foodproof® StarPrep Two Kit

The foodproof StarPrep Two Kit provides rapid DNA extraction from bacteria, yeast, and mold for direct PCR applications. This streamlined kit handles enrichment cultures, direct samples, and filtered water samples, processing up to 1 mL sample volume in a single tube. The lysis buffer eliminates hazardous organic extractions and chaotropic agents while minimizing cross-contamination risks. Designed for 96 reactions, the kit delivers high-quality DNA suitable for PCR detection of Listeria, Clostridium, Legionella, beer-spoiling bacteria, spoilage yeast, Aspergillus and others. Save time with reduced handling steps and get reliable results for food safety testing.

42 mL
KIT230177
Depending on the extraction protocol, additional reagents and devices are used:

Additional Reagents

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 Reagent D

Reagent D eliminates DNA from dead cells to prevent false-positive PCR results in food safety testing. This light-sensitive reagent penetrates dead cell membranes and binds to DNA upon light exposure, blocking PCR amplification. Validated for testing Enterobacteriaceae, Cronobacter, Salmonella, Legionella, and Vibrio in various food matrices including instant milk formula and dairy products. Compatible with Hygiena's DNA isolation kits, Reagent D ensures accurate detection of viable microorganisms in food safety and environmental monitoring applications.

KIT230001
Rinse Buffer

 Rinse Buffer

The Rinse Buffer is designed for optimal recovery of microbes from filter membranes.

100 mL
KIT230012
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 Suspension Buffer

The Suspension Buffer simplifies bacterial colony preparation for PCR testing with a streamlined, single-tube protocol. This specialized buffer enables direct suspension of colonies from agar plates without hazardous organic extractions or chaotropic agents. The extract maintains cell viability for additional analyses like serotyping or DNA sequencing. The efficient process delivers PCR-ready samples while reducing handling steps and exposure to hazardous materials. Perfect for quality control labs needing fast, reliable sample preparation for food safety and microbial identification testing.

40 mL
KIT230178

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